Comments on: DNA Replicator in Your Pocket http://www.eyeondna.com/2007/05/02/dna-replicator-in-your-pocket/ How will it change your life? Mon, 19 Jul 2010 13:56:21 +0800 http://wordpress.org/?v=2.8.4 hourly 1 By: Hsien-Hsien Lei, PhD http://www.eyeondna.com/2007/05/02/dna-replicator-in-your-pocket/comment-page-1/#comment-57 Hsien-Hsien Lei, PhD Fri, 04 May 2007 15:41:57 +0000 http://www.eyeondna.com/2007/05/02/dna-replicator-in-your-pocket/#comment-57 <b>James</b>: Thank you so much! I'm afraid I'm simply addicted to science blogging. Can't explain why else I do it.... Oh, the compliments help too! :D James: Thank you so much! I’m afraid I’m simply addicted to science blogging. Can’t explain why else I do it…. Oh, the compliments help too! :D

]]> By: James Wachai http://www.eyeondna.com/2007/05/02/dna-replicator-in-your-pocket/comment-page-1/#comment-55 James Wachai Fri, 04 May 2007 10:29:55 +0000 http://www.eyeondna.com/2007/05/02/dna-replicator-in-your-pocket/#comment-55 I must, also, jump into this bandwagon and say that Hsien is a very passionate writer. I am yet to meet a scientist with a passion to write as Hsien. She has demystified science blogging. Hsien is the mirror through which all science bloggers should see their future. I must, also, jump into this bandwagon and say that Hsien is a very passionate writer. I am yet to meet a scientist with a passion to write as Hsien. She has demystified science blogging. Hsien is the mirror through which all science bloggers should see their future.

]]> By: Hsien-Hsien Lei, PhD http://www.eyeondna.com/2007/05/02/dna-replicator-in-your-pocket/comment-page-1/#comment-47 Hsien-Hsien Lei, PhD Thu, 03 May 2007 08:35:06 +0000 http://www.eyeondna.com/2007/05/02/dna-replicator-in-your-pocket/#comment-47 <b>Brian</b>: lol That's a good point. But the little gadget is still quite novel and is a good start! I'd like to know what the prep is like. Just imagine trying to control contamination, minuscule samples, and reagents in the field. *headache* Brian: lol That’s a good point. But the little gadget is still quite novel and is a good start! I’d like to know what the prep is like. Just imagine trying to control contamination, minuscule samples, and reagents in the field. *headache*

]]> By: Brian http://www.eyeondna.com/2007/05/02/dna-replicator-in-your-pocket/comment-page-1/#comment-44 Brian Wed, 02 May 2007 18:16:47 +0000 http://www.eyeondna.com/2007/05/02/dna-replicator-in-your-pocket/#comment-44 Comparing 20 minute PCR to a few hours is kind of misleading. A PCR that takes a 2 hours doesn't have ramping time (the time it takes to go from one temp to the next) of 1 hour and 40 minutes. I'm sure their system is faster since the ramping time seems to be close to nothing, but I think they're comparing the minimum time on their machine (20 cycles with a 15 second extension?) with a typical PCR on a current machine. Heck, I can do a 20 minute PCR right now if I add enough DNA and have a short enough product. Comparing 20 minute PCR to a few hours is kind of misleading. A PCR that takes a 2 hours doesn’t have ramping time (the time it takes to go from one temp to the next) of 1 hour and 40 minutes. I’m sure their system is faster since the ramping time seems to be close to nothing, but I think they’re comparing the minimum time on their machine (20 cycles with a 15 second extension?) with a typical PCR on a current machine.

Heck, I can do a 20 minute PCR right now if I add enough DNA and have a short enough product.

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